[Colin]

I googled DNP ie 2,4-dinitrophenol and found this so I'd like to find out if this is a second rate version of DNP WRT fat oxidation.I'm guessing it's not,otherwise we would have heard of it by now but perhaps in conjunction with algae(study below which also mentions ATP but I can't decipher much of it) we could get DNP-esque effects,hopefully enough to burn fat in an equivalent amount as 200-400mg DNP per day.

DNP is no longer readily available and if sources can be found,they are quite high.This is ompared to everybody's favorite chem warehouse supplier,which apparently never have DNP in stock so if the 2-6 works as an uncoupler as well with a side effect profile on par with DNP.....then I'd use it.

Any thoughts as to whjat this powder is and does within the body would be much appreciated,I'd like to get a decent discussion going.

2,6-Dinitrophenol
NMR MS MSDS
Catalog Number 4116
CAS Registry number 573-56-8
Molecular Formula C6H4N2O5
Formula Weight 184.11
Specification

Appearance: White crystalline powder
Assay: 98%(MIN,GC)
Melting point: 61-63 °C(lit.)
Moisture:0.5%(Max)
Hazard Symbol N: Dangerous for the environment
T: Toxic
Risk Code R23/24/25;33;51/53
Safety Description S28;37;45;61
Transport information UN 1320
Availability Up to kgs
Application Intermediate in organic syntheses




Here's a study on it with algae,I'm posting it b/c of all the talk over fucoxathin and apparent potential astaxathin also has in fat loss.

Oxygen-dependent proton efflux in cyanobacteria (blue-green algae).
Scherer S, Stürzl E, Böger P.

The oxygen-dependent proton efflux (in the dark) of intact cells of Anabaena variabilis and four other cyanobacteria (blue-green algae) was investigated. In contrast to bacteria and isolated mitochondria, an H+/e ratio (= protons translocated per electron transported) of only 0.23 to 0.35 and a P/e ratio of 0.8 to 1.5 were observed, indicative of respiratory electron transport being localized essentially on the thylakoids, not on the cytoplasmic membrane. Oxygen-induced acidification of the medium was sensitive to cyanide and the uncoupler carbonyl cyanide m-chlorophenylhydrazone. Inhibitors such as 2,6-dinitrophenol and vanadate exhibited a significant decrease in the H+/e ratio. After the oxygen pulse, electron transport started immediately, but proton efflux lagged 40 to 60 s behind, a period also needed before maximum ATP pool levels were attained. We suggest that proton efflux in A. variabilis is due to a proton-translocating ATP hydrolase (ATP-consuming ATPase) rather than to respiratory electron transport located on the cytoplasmic membrane.

PMID: 6327614 [PubMed - indexed for MEDLINE]

[Jakeshorts]

I was under the notion that it's the chemical compound name of DNP. I've used this nomenclature to search for this elusive son of a bitch myself.

[Colin]

2,4 and 2,6 dini are not the same,hence 2,6 is not what we refer to as DNP.

Here's something I just found,I've just started looking for studies on 2,6 showing fat loss....

Role of tissue hypermetabolism in stimulation of ventilation by dinitrophenol.
Levine S.

Several authors have hypothesized that tissue hypermetabolism accounts for increases in ventilation (VE) elicited by 2,4-dinitrophenol. However, some data in the literature indicate that stimulation of VE by isomers of dinitrophenol is unrelated to tissue metabolic rate. To test this latter concept, we compared three different isomers of dinitrophenol (i.e., 2,4-dinitrophenol (2,4-DNP), 2,5-dinitrophenol (2,5,-DNP), 2,6-dinitrophenol (2,6-DNP) with respect to stimulation of VE and with respect to stimulation of oxygen consumption (VO2). In all experiments, 3-4 mg/kg of one dinitrophenol isomer was administered to chloralose anesthetized dogs by intra-arterial infusion. 2,4-DNP elicited large increments in both VE and VO2, 2,6-DNP elicited moderate increments in both VE and VO2, whereas 2,5-DNP elicited small increments in both VE and VO2. These observations demonstrate a correlation between ventilatory and metabolic changes affected by isomers of dinitrophenol. Accordingly, these results are consistent with the hypothesis that ventilatory stimulation by congeners of dinitrophenol is related to tissue hypermetabolism.

PMID: 19413 [PubMed - indexed for MEDLINE]

[Jakeshorts]

wow.... I completely missed the numbers.. I just saw that they were there and assumed it was 2,4. I'm still upset I didn't make the man crush list. I'll heal... with time.


Might i speculate that 2,6 is the pesticide?

[Colin]

No problem,amigo.

In retrospect I should have made a note in the title indicating that 2,4 and 2,6 wereseperarte entities and not to be lumped together under the slassification of DNP.The former we know is DNP,the latter I'm lost on.


2,4 is certainly a pesticide as that's been an intended use of DNP for a long time but today's inadvertent find of 2,6 if the first I've heard of something that MAY exert fat oxidation along the lines of 2,4 dinitrophenol.

Obviously they are both related,what I'm trying to find out is what they have in common and how the effect of fat loss can be duplicated via 2,6.

Perhaps one could obtain (This is easy and cheap to obtain opposed to DNP) the 2,6 and play mad scientist in their garage by converting the 2,6 to the 2,4.The study below seems to suggest is possible but this is over my head.



Anaerobic aquifer transformations of 2,4-dinitrophenol under different terminal electron accepting conditions.
Krumholz LR, Suflita JM.

Department of Botany and Microbiology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019-0245, USA. krumholz@ou.edu

We evaluated the susceptibility of 2,4-dinitrophenol (2,4-DNP) and 2,4-diaminophenol to anaerobic biodegradation in aquifer slurries. Aquifer microorganisms depleted 2,4-DNP at rates of 25, 9 and 0.4 microM/day under methanogenic, sulfate-reducing and nitrate-reducing conditions, respectively. Rates of abiotic, 2,4-DNP loss in autoclaved control incubations were 7.2, 6.2 and 0.95 microM/day respectively. Abiotic, 2,4-DNP reduction was especially important as the first step in its transformation. 2-Amino-4-nitrophenol was produced by this process, but this compound was further metabolized in methanogenic and sulfate-reducing aquifer slurries. This partially reduced compound persisted in autoclaved controls and in the nitrate-reducing aquifer slurries. Aquifer slurries incubated with either 2,4-DNP or 2,4-diaminophenol produced methane when incubated with no other electron acceptor suggesting that mineralization had occurred under these conditions. In parallel experiments, aquifer slurries amended with 2,6-dinitrophenol or picric acid did not produce methane at levels above the substrate unamended controls.

PMID: 16887615 [PubMed]

[Jakeshorts]

judging by the post above it seems 2,6 is picric acid which is a TRInitrophenol

what wiki says about it:

Picric acid is the chemical compound more formally called 2,4,6-trinitrophenol (TNP). This a yellow crystalline solid is one of the most acidic phenols. Like other highly nitrated compounds such as TNT, picric acid is an explosive


if it IS this explosive picric acid I would imagine it would be harder to obtain

[Colin]

2,6 is a white powder,not yellow.As illustrated in the chemical profile in my first page,which was taken from the order form of the supplier.

[jae23]

If I remember my O-chem correctly 2,6- and 2,4- dinitro's are isomers born from the same chemical reaction. Thermodynamics favor the formation of 2,6 though and the isomers are then isolated via chromatography (GLC/HPLC). You can block addition to the 6 carbon and yield a higher percentage of 2,4 but either way these types of synthesis/reactions are usually HIGHLY exothermic and only very small quanitities should be produced if not working in a lab with equipment suited for this type of work. I remember doing a similar reaction in O-chem lab at college and it was a very difficult reaction to control. I even got some 2,4,6 product. My point is these types of reactions are very difficult to control and much more difficult to get the desired product in high purity and amount so I would say to think twice before opening up your O-chem book and playing mad scientist.

[thecrownedone]

With 2,6 being white, we may have the favorable circumstance of being able to rid ourselves of "DNP jaundice," as it were. Does 2,6 sublimate as well?

[Colin]

jae23, on Jan 2 2008, 01:46 PM, said:

If I remember my O-chem correctly 2,6- and 2,4- dinitro's are isomers born from the same chemical reaction. Thermodynamics favor the formation of 2,6 though and the isomers are then isolated via chromatography (GLC/HPLC). You can block addition to the 6 carbon and yield a higher percentage of 2,4 but either way these types of synthesis/reactions are usually HIGHLY exothermic and only very small quanitities should be produced if not working in a lab with equipment suited for this type of work. I remember doing a similar reaction in O-chem lab at college and it was a very difficult reaction to control. I even got some 2,4,6 product. My point is these types of reactions are very difficult to control and much more difficult to get the desired product in high purity and amount so I would say to think twice before opening up your O-chem book and playing mad scientist.

Thanks for the low down.

I was just throwing the idea out there to see if it was remotely plausible to home brew some 2,4.I was thinking of something like how easy it is to get meth from pseudoephedrine but as you know what you're talking about,I won't attempt to do anything along those lines.

Do you know if 2,6 has any lipid oxidation properties?

What I'm trying to find out is if the 2,6 is comparable to the 2,4 in this respect.Obviously it wouldnt; be as potent mg per mg as the 2,4 or we would all know about this by now.Nonetheless,I'd really like to know if the 2,6 is worthwhile to use as a fat loss aid,any help you can give me on this would be much appreciated.